Use of reverse transcriptase PCR to subtype N1 to N9 neuraminidase genes of avian influenza viruses.
نویسندگان
چکیده
Reverse transcriptase PCR designed to amplify the N1 to N9 neuraminidase (NA) genes of avian influenza viruses detected 118 of the 119 NA genes tested (99.2%) in a subtype-specific manner. This technique successfully subtyped all 167 recent avian influenza viruses isolated from birds. Subtype specificity was confirmed by sequence analyses of all 285 PCR products.
منابع مشابه
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Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans. Highly pathogenic avian influenza viruses (HPAI) H5N1 were first identified in Southeast Asia in 1996 and spread to four continents over the following years. The viruses have caused high mortality in chickens and various bird species ...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 47 7 شماره
صفحات -
تاریخ انتشار 2009